Coding

Part:BBa_M45155:Design

Designed by: Logan Sherman   Group: UtahState_BE5930_-_S14   (2016-04-20)


M. tuberculosis antigen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 870
    Illegal AgeI site found at 327
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 627


Design Notes

The DNA was optimized for protein production in E. coli.


Source

Sourced from the amino acid sequence of antigen MPT32 produced by M. tuberculosis

References