Coding
Part:BBa_M45155:Design
Designed by: Logan Sherman Group: UtahState_BE5930_-_S14 (2016-04-20)
M. tuberculosis antigen
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 70
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 730
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 870
Illegal AgeI site found at 327 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 627
Design Notes
The DNA was optimized for protein production in E. coli.
Source
Sourced from the amino acid sequence of antigen MPT32 produced by M. tuberculosis